Structure and Composition of Gap Junctions


Gap junctions are specialized intercellular channels that allow for direct communication and exchange of small molecules between adjacent cells. They play a crucial role in various physiological processes, including cell signaling, coordination of cellular activities, and tissue homeostasis. In this article, we will explore the structure and composition of gap junctions, shedding light on their importance in cellular communication.

The Basic Structure of Gap Junctions

Gap junctions are composed of clusters of proteins called connexins. Connexins are transmembrane proteins that form the channels through which molecules can pass between cells. Each connexin protein consists of four transmembrane domains, two extracellular loops, one intracellular loop, and cytoplasmic N- and C-termini. When connexins from adjacent cells come together, they form a channel known as a connexon or hemichannel.

Connexons and Gap Junction Channels

Connexons are hexameric structures formed by the assembly of six connexin proteins. In most cases, connexons are composed of identical connexin subunits, referred to as homomeric connexons. However, heteromeric connexons, composed of different connexin subunits, can also exist. Each connexon is located in the plasma membrane of a single cell and can dock with a connexon from an adjacent cell to form a complete gap junction channel.

When connexons from neighboring cells align and dock, the connexin channels within each connexon align as well, creating a continuous pore between the two cells. This pore allows for the direct exchange of ions, metabolites, and small signaling molecules, such as cyclic AMP and calcium ions, between the cytoplasm of the two connected cells.

Regulation of Gap Junctions

The opening and closing of gap junction channels are tightly regulated to control the flow of molecules between cells. Connexin proteins can be phosphorylated or dephosphorylated, which affects their conformation and the permeability of the gap junction channels. Various intracellular signaling pathways, such as calcium signaling and protein kinase cascades, can modulate the phosphorylation status of connexins and regulate the activity of gap junctions.

Additionally, the composition of connexins within connexons can also influence the properties of gap junction channels. Different connexin isoforms have distinct biophysical properties and can confer specific functional characteristics to the gap junction channels. The expression of specific connexin isoforms can vary among different cell types and tissues, allowing for the specialization of gap junction-mediated communication.

Localization and Distribution of Gap Junctions

Gap junctions are found in various tissues and cell types throughout the body. They are particularly abundant in tissues that require coordinated cellular activities, such as cardiac muscle, smooth muscle, and the nervous system. In these tissues, gap junctions facilitate the rapid transmission of electrical signals and allow for synchronized contractions or coordinated responses to stimuli.

The distribution of gap junctions within tissues can vary depending on the specific physiological requirements of the cells. In some cases, gap junctions are evenly distributed along the plasma membrane of adjacent cells, forming gap junction plaques. In other instances, they may be concentrated at specific regions, such as cell-cell interfaces or specialized structures like intercalated discs in cardiac muscle.


Gap junctions are essential components of cellular communication, enabling the direct exchange of molecules between adjacent cells. The structure and composition of gap junctions, primarily composed of connexin proteins, allow for the formation of intercellular channels that facilitate the rapid and coordinated transmission of signals. The regulation, localization, and distribution of gap junctions play critical roles in various physiological processes and are crucial for the proper functioning of tissues and organs. Understanding the structure and composition of gap junctions provides valuable insights into the mechanisms underlying intercellular communication and highlights their significance in maintaining cellular homeostasis.

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